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Physiological measurements were obtained from controlled laboratory incubations following standardised protocols. After an acclimation period, faecal pellets were collected using a vacuum-based siphoning system and subsequently used to determine sinking velocity in still-water columns by timing their descent over fixed distances. Pellet lengths were measured from microscope images analysed in ImageJ. Respiration rates were derived from changes in dissolved oxygen concentration, which were continuously monitored using calibrated Firesting optical oxygen sensors placed in stirred incubation chambers. Clearance rates were calculated from changes in particle concentration measured with a Coulter counter, and faecal pellet production was quantified from the dry mass and carbon content of pellets collected over known time intervals. All physiological rates were standardised to organism dry weight and carbon content, determined after drying soft tissues. Quality control procedures included calibration of sensors prior to measurements, the use of replicates and procedural controls, verification of calculation procedures, and the dataset underwent standard quality checks before harmonisation and archiving.

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ISO 19115:2003/19139